| Transit agency | City | Vehicles operated directly | Website |
|---|---|---|---|
| Metropolitan Transit Authority | Madison | 196 | Official site |
| City of Memphis | Memphis | 135 | Official site |
| South Central Tennessee Development District | Mt Pleasant | 124 | Official site |
| Mid-Cumberland Human Resource Agency | Nashville | 109 | Official site |
| Upper-Cumberland Human Resource Agency | Cookeville | 102 | Official site |
| East Tennessee Human Resource Agency, Inc. | Knoxville | 83 | Official site |
| Southeast Tennessee Human Resource Agency-Rural Division | Dunlap | 83 | Official site |
| Chattanooga Area Regional Transportation Authority | Chattanooga | 76 | Official site |
| City of Knoxville | Knoxville | 64 | Official site |
| First Tennessee Human Resource Agency | Johnson City | 61 | Official site |
| Northwest Tennessee Human Resource Agency | Martin | 46 | Official site |
| Southwest Human Resource Agency | Henderson | 41 | Official site |
| Delta Human Resource Agency | Covington | 30 | Official site |
| City of Clarksville | Clarksville | 29 | Official site |
| Knoxville-Knox County Community Action Committee | Knoxville | 29 | Official site |
| City of Johnson City | Johnson City | 25 | Official site |
| City of Pigeon Forge | Pigeon Forge | 19 | Official site |
| Jackson Transit Authority | Jackson | 13 | Official site |
| Southeast Tennessee Human Resource Agency | Dunlap | 12 | Official site |
| City of Kingsport | Kingsport | 11 | Official site |
| City of Bristol Tennessee | Bristol | 7 | Official site |
| City of Gatlinburg | Gatlinburg | 6 | Official site |
| City of Murfreesboro | Murfreesboro | 6 | Official site |
| Franklin Transit Authority | Franklin | 0 | Official site |
| Regional Transportation Authority | Madison | 0 | Official site |
Largest first by vehicles operated directly, as each employer reports it to its federal registry.
Source: Federal Transit Administration, National Transit Database 2024 — Reporter Agency Information. Data captured September 30, 2026.