| Transit agency | City | Vehicles operated directly | Website |
|---|---|---|---|
| Transit Authority of Omaha | Omaha | 101 | Official site |
| City of Lincoln | Lincoln | 74 | Official site |
| Kimball County Public Transit | Kimball | 21 | Official site |
| Ponca Tribe of Nebraska | Niobrara | 16 | Official site |
| Cedar County Transit | Hartington | 11 | Official site |
| Scotts Bluff Public Transit | Gering | 9 | Official site |
| City of North Platte | North Platte | 8 | Official site |
| Omaha Tribe of Nebraska | Macy | 7 | — |
| Chase County | Imperial | 5 | Official site |
| City of Alliance | Alliance | 5 | Official site |
| City of Columbus Transit | Columbus | 5 | Official site |
| Saline County Area Transit | Western | 5 | Official site |
| Senior Information Center York | York | 5 | Official site |
| Butler County Senior Service Program | David City | 4 | Official site |
| City Of Sidney | Sidney | 4 | Official site |
| Garden County | Oshkosh | 4 | Official site |
| Seward County Public Transit | Seward | 4 | Official site |
| Wayne Public Transit | Wayne | 4 | Official site |
| Callaway District Hospital | Callaway | 3 | Official site |
| City of Chadron | Chadron | 3 | Official site |
| City of Fremont | Fremont | 3 | Official site |
| City of Neligh | Neligh | 3 | Official site |
| Lancaster Co Rural Transit | Lincoln | 3 | Official site |
| Ogallala Transit System | Ogallala | 3 | Official site |
| City of Crawford | Crawford | 2 | — |
| City Of McCook Public Transit | Mc Cook | 2 | Official site |
| Hooker Co | Mullen | 2 | Official site |
| Loup City | Loup City | 2 | Official site |
| Morrill County Handi Bus | Bridgeport | 2 | Official site |
| Perkins County Public Transit | Grant | 2 | — |
| Phelps County Senior Citizen | Holdrege | 2 | Official site |
| Winnebago Tribe of Nebraska | Winnebago | 2 | — |
| City of Benkelman | Benkelman | 1 | Official site |
| City of Plainview Handivan | Plainview | 1 | Official site |
| City Of Tecumseh | Tecumseh | 1 | Official site |
| Grant County | Hyannis | 1 | — |
| Harlan Co. Public Transit | Alma | 1 | — |
| Santee Sioux Nation | Niobrara | 1 | — |
| Saunders County Transportation | Wahoo | 1 | Official site |
| Schuyler | Schuyler | 1 | Official site |
| City of Grand Island | Grand Island | 0 | Official site |
| Nebraska Department of Transportation | Lincoln | 0 | Official site |
Largest first by vehicles operated directly, as each employer reports it to its federal registry.
Source: Federal Transit Administration, National Transit Database 2024 — Reporter Agency Information. Data captured September 30, 2026.