| Transit agency | City | Vehicles operated directly | Website |
|---|---|---|---|
| Bi-State Development Agency of the Missouri-Illinois Metropolitan District | Saint Louis | 394 | Official site |
| Kansas City Area Transportation Authority | Kansas City | 134 | Official site |
| Cape Girardeau County Transit Authority | Cape Girardeau | 28 | Official site |
| City of Columbia | Columbia | 24 | Official site |
| City of Springfield | Springfield | 22 | Official site |
| City of Jefferson | Jefferson City | 18 | Official site |
| City of St. Joseph, Missouri | Saint Joseph | 16 | Official site |
| City of Joplin | Joplin | 8 | Official site |
| Southeast Missouri State University | Cape Girardeau | 5 | Official site |
| City of Clinton | Clinton | 4 | Official site |
| City of Excelsior Springs | Excelsior Springs | 4 | Official site |
| City of West Plains | West Plains | 4 | Official site |
| City of Lamar | Lamar | 3 | Official site |
| City of Mount Vernon | Mount Vernon | 3 | Official site |
| City of Bloomfield | Bloomfield | 2 | — |
| City of Carthage | Carthage | 2 | Official site |
| City of El Dorado Springs | El Dorado Springs | 2 | Official site |
| City of Houston | Houston | 2 | Official site |
| City of New Madrid | New Madrid | 2 | Official site |
| City of Nevada | Nevada | 1 | Official site |
| New Bourbon Regional Port Authority | Perryville | 1 | Official site |
| City of Independence | Independence | 0 | Official site |
| Kansas City, City of Missouri | Kansas City | 0 | Official site |
| Loop Trolley Transportation Development District | Saint Louis | 0 | Official site |
Largest first by vehicles operated directly, as each employer reports it to its federal registry.
Source: Federal Transit Administration, National Transit Database 2024 — Reporter Agency Information. Data captured September 30, 2026.