| Transit agency | City | Vehicles operated directly | Website |
|---|---|---|---|
| Birmingham-Jefferson County Transit Authority | Birmingham | 74 | Official site |
| City of Mobile | Mobile | 40 | Official site |
| City of Huntsville, Alabama | Huntsville | 36 | Official site |
| Cullman County Commission | Cullman | 30 | Official site |
| Baldwin County Commission | Bay Minette | 28 | Official site |
| City of Montgomery | Montgomery | 25 | Official site |
| Lee-Russell Council of Governments | Opelika | 20 | Official site |
| Alabama Tombigbee Regional Planning Commission | Camden | 15 | Official site |
| Tuscaloosa County Parking and Transit Authority | Tuscaloosa | 15 | Official site |
| Northwest Alabama Council of Local Governments | Muscle Shoals | 14 | Official site |
| Southeast Alabama Regional Planning and Development Commission | Dothan | 13 | Official site |
| City of Gadsden | Gadsden | 12 | Official site |
| Dekalb County Commission | Fort Payne | 12 | Official site |
| Lawrence County Aging-Rural Transit System | Moulton | 12 | — |
| St. Clair County Commission | Ashville | 12 | Official site |
| Autauga County | Prattville | 11 | Official site |
| Chilton County Commission | Clanton | 10 | Official site |
| North Central Alabama Regional Council of Governments | Decatur | 9 | Official site |
| Escambia County Commission | Brewton | 8 | Official site |
| Jackson County | Scottsboro | 7 | Official site |
| Pike Area Transit System | Troy | 7 | Official site |
| Blount County Commission | Oneonta | 6 | Official site |
| Macon County Commission | Tuskegee | 6 | Official site |
| Madison County Commission | Huntsville | 6 | Official site |
| Walker County Commission | Jasper | 6 | Official site |
| City of Guntersville | Guntersville | 5 | Official site |
| Etowah County Commission | Gadsden | 5 | Official site |
| Washington County | Chatom | 5 | Official site |
| City of Eufaula | Eufaula | 4 | Official site |
| Covington County | Andalusia | 4 | Official site |
| Poarch Band of Creek Indians | Atmore | 3 | — |
| City of Fayette | Fayette | 2 | Official site |
| East Alabama Regional Planning and Development Commission | Anniston | 0 | Official site |
| Regional Planning Commission of Greater Birmingham | Birmingham | 0 | Official site |
Largest first by vehicles operated directly, as each employer reports it to its federal registry.
Source: Federal Transit Administration, National Transit Database 2024 — Reporter Agency Information. Data captured September 30, 2026.